Device

Part:BBa_K2719013

Designed by: Juan Carlos Rueda Silva & Arantxa Karam Coppola   Group: iGEM18_TecCEM   (2018-10-10)


Blue Chromoprotein and RFP combined reporter

Combined reporter basic model, this part incorporates a constitutively expressed blue chromoprotein cassette (BBa_K592015) and a Tet repressible RFP expression cassette (BBa_I13521). This part serves as a model for the development of multiple reporter systems and how a colony would look when there are multiple reporter proteins and expressed or repressed at the same time. This part improves the understanding of multiple reporter systems and the interactions of two reporter proteins, letting to the understanding of the observations of those systems. Also, was made as a control for collagen (BBa_K2719011) that has the two reporter genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 938
    Illegal NheI site found at 961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None